Val-->Ala mutations selectively alter helix-helix packing in the transmembrane segment of phage M13 coat protein.

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Val-->Ala mutations selectively alter helix-helix packing in the transmembrane segment of phage M13 coat protein.

Val-->Ala mutations within the effective transmembrane segment of a model single-spanning membrane protein, the 50-residue major coat (gene VIII) protein of bacteriophage M13, are shown to have sequence-dependent impacts on stabilization of membrane-embedded helical dimeric structures. Randomized mutagenesis performed on the coat protein hydrophobic segment 21-39 (YIGYAWAMV-VVIVGATIGI) produced...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1993

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.90.24.11648